PULSAR

PULSAR (an acronym of Protein Unfolding for Ligand Stabilisation and Ranking, formerly ionMS) is specialised software for the processing and analysis of ion mobility-mass spectrometry data. PULSAR can import data directly from Waters RAW files, annotated with appropriate meta data, and enables:

• Viewing of ion mobility mobiligrams
• Basic deconvolution and charge state assignment for native mass spectra
• Calculation of calibrations for travelling-wave ion mobility collision cross section (CCS) measurements
• Automated CCS measurements of protein and small molecule ions
• Extraction of mobiligram regions for ion mobility-based separation
• Enhanced analysis of gas-phase hydrogen-deuterium exchange experiments
• Generation of gas-phase protein unfolding plots
• Comprehensive analysis and calculation of gas phase behaviour and stability of ions

PULSAR is freely available as a Windows-packaged distribution upon request to Tim.